Cell Reprogramming: Methods and Protocols

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Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

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Authoritative and cutting-edge, this book is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians and animal care technicians involved with reprogramming, nuclear transfer and transgenesis. User Name Password Sign In.

3 Methods for Passaging of Pluripotent Stem Cells

Cell Reprogramming. Methods and Protocols Cell Reprogramming.

Viral Vectors

Methods and Protocols. Edited by P. Poleganov, M. Efficient reprogramming of human fibroblasts and blood-derived endothelial progenitor cells using nonmodified RNA for reprogramming and immune evasion.

Stem Cell Reprogramming – Leaner, Quicker, Cheaper, Better

Gene Ther. Yoshida, Y. Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell 5 , — Beers, J. Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions. Fusaki, N. Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendaivirus, an RNA virus that does not integrate into the host genome. Japan Acad. B 85 , — Schlaeger, T. A comparison of non-integrating reprogramming methods. Churko, J. Transcriptomic and epigenomic differences in human induced pluripotent stem cells generated from six reprogramming methods.

Integrating iPSC delivery methods

Okita, K. A more efficient method to generate integration-free human iPS cells.

Methods 8 , — Yoshioka, N. Cell Stem Cell 13 , — Warren, L. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7 , — Mandal, P. Reprogramming human fibroblasts to pluripotency using modified mRNA.

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Kogut, I. High-efficiency RNA-based reprogramming of human primary fibroblasts.

Stem Cell Reprogramming - Applications

Melin, J. Microfluidic large-scale integration: the evolution of design rules for biological automation. Qin, D. Soft lithography for micro- and nanoscale patterning. Characterization of pluripotent stem cells. Cacchiarelli, D.

Equipment Required

Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency. Mali, P. Stem Cells 28 , — Zhu, S. Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Ichida, J.